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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
WAY-331974
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
901750-93-4
- 规格:
5mg
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文献和实验.niox.2009.01.002 (2009). 11. Xiao, Y. & Truskey, G.A. Effect of receptor-ligand affinity on the strength of endothelial cell adhesion. Biophys. J. 71, 2869-2884, doi:10.1016/S0006-3495(96)79484-5 (1996). 12. Conant, C.G., Schwartz, M
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
and ballssterile Tissue retractorsTissue scissorsLactated Ringer's solution (LR) containing Penicillin/StreptomycinFiberoptic light sourceVirus stockAutomated microinjector1-5 ml micropipettes3-0 Dexon sutureTissue stapler1. Mix ketamine and xylazine 1:1 in a 1 ml
in a thermocycler. Heat sample to 95°C, then add 0.5 -1 ul of enzyme (Taq, Tli, Pfu etc.). Add a few drops of mineral oil.3.Start the PCR cycles according the following schemes:(1) denaturation - 94 ° C, 30-90 sec.(2) annealing - 55 °C (or -5° Tm), 0.5-2 min
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