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screws, 1 pair of tweezers, one 75 x 25 x 1 mm glass slide (or other desired cell surface material), and 2 surgical towels.     6)      Place sterile towels into laminar flow hood. Place the flow circuit, flow chamber, 4 x 4-way stopcocks, 1 x 1-way

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groups have used siRNA stems ranging from 21 nucleotides-long (4-5) to 25-29 nucleotides-long (11). It is found that hairpin siRNAs with these various stem lengths all function well in gene silencing studies. Length and Sequence of the Loop Linking Sense

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

6). 5. S-gal LB powder (Sigma, St. Louis, MO) (see Note 7). 6. Solution I: 50 mM glucose, 25 mM Tris–HCl (pH 8.0), and10 mM EDTA (pH 8.0). Filter sterilized. Store at 4°C. 7. Solution II: 0.1 M NaOH and 1% SDS. Store at 4°C. 8. Solution