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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
Dimethylphenylfluorosilane
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
454-57-9
- 规格:
250mg
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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
100 mM dNTP Set PCR grade (Life Technologies; Cat # 10297-018) Primers: Poly d(T) [20-mers] 2.5 mg/mL (avoids labeling the gDNA and rRNA.) Anchored poly d(T) primers (Archaic) Random Hexamer primers (3mg/mL) (Life
Native Chromatin Preparation and Illumina/Solexa Library Construction
, including the Genome Analyzer (Solexa/Illumina), 454 (Roche), and ABI-SOLiD (Applied Biosystems). This protocol describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using
filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
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