季酮酸;541-57-1;95%;S24802-250mg

ORNL MICROARRAY HYBRIDIZATION PROTOCOLS

100 mM dNTP Set PCR grade (Life Technologies; Cat # 10297-018) Primers:   Poly d(T) [20-mers] 2.5 mg/mL (avoids labeling the gDNA and rRNA.)                 Anchored poly d(T) primers            (Archaic) Random Hexamer primers (3mg/mL) (Life

Single tube confirmation PCR protocol

(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants.   2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution

Differential Display of RNAs

inactivate MMLV RT by incubation for 5 min at 95 °C use immediately for PCR amplification or store at -20 °C   III. PCR reaction all PCR s should be done in doublets and always run in the same machine (to minimize