δ-hexatoxin-Mg1a;0;K21058-500μ

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

6.5 and 2µl of 10mg/ml Proteinase K and incubated in 45°C water bath for 1 hour;Recover DNA/RNA by Phenol/Chloroform extraction and ethanol precipitation (note 6); ;Re-suspend the pelleted DNA in ~30µl of water and perform PCR or real-time PCR analysis

核酸、蛋白技术参数资料、分子量标准

48502（双链环状）3.0×107pBR3224363（双链）2.8×10628SrRNA48001.6×10623SrRNA37001.2×10618SrRNA19006.1×10519SrRNA17005.5×1055SrRNA1203.6×104tRNA（大肠杆菌）752.5×1043．常用核酸蛋白换算数据　　（1）重量换算　　1μg=10-6g　　　　　 1pg=10-12g　　1ng=10-9g 　　　　　1fg=10-15g　　（2）分光光度换算：　　1A260双链DNA=50μg/ml　

Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sar

to obtain 200–500 μg/L of yeast culture of a 50% pure SERCA1a that displays an ATPase activity similar to that of the native rabbit Ca2+ -ATPase. To succeed in crystallization, an additional size exclusion chromatography step was necessary. This step