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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
Vacuum pump oil
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
64741-88-4
- 规格:
1L
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文献和实验Large Scale Tubulin Preparation
3.5 liters of 10X CB for 10-12 brain prep. GTP : Sigma Type IIS- # G-8752 ATP : Sigma Grade 1- # A-2383 Glycerol : 2-3L prewarmed to 37¡C (store overnight in 37¡C incubator) III. Pouring a 1L Phosphocellulose (PC) Column
Large Scale Tubulin Preparation
Grade 1- # A-2383 Glycerol : 2-3L prewarmed to 37¡C (store overnight in 37¡C incubator) III. Pouring a 1L Phosphocellulose (PC) Column Resin : Whatman P11 Cellulose Phosphate -- fibrous cation exchanger (1 gram of PC swells
for equilibration, running and storage of PC column CB + 1 M KCl: Need ~10 liters for prewashing and eluting the PC column To make 1L of 10X CB: 151.2 grams PIPES, free acid 3.8 grams EGTA 2 ml of 1 M MgCl2 pH with KOH to pH 6.75
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