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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
Vacuum pump oil
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
64741-88-4
- 规格:
1L
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
will work! Keep the membrane wet and wrap in Saran wrap. Add radioactive markings for orientation of filmlater Expose to film. 2.Cut out band of interest,re-wet in methanol,rinse well in water and place in a screw-cap tube containing 150 µl 5.7 NHCl
Phosphoamino acid analysis:Mark Kamps method
Phosphoamino acid analysis:Mark Kamps's method 1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P .
Large Scale Tubulin Preparation
3.5 liters of 10X CB for 10-12 brain prep. GTP : Sigma Type IIS- # G-8752 ATP : Sigma Grade 1- # A-2383 Glycerol : 2-3L prewarmed to 37¡C (store overnight in 37¡C incubator) III. Pouring a 1L Phosphocellulose (PC) Column
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