- ￥410
- 源叶
- 中国
- R32372-4*250ul
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- 规格：
4*250ul
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文献和实验RNA Analysis by Nuclease Protection
) 0.5‐ml RNase‐free microcentrifuge tubes 95° to 100°C heating block or water bath Additional reagents
Nested Deletions Using Exonuclease-III and Mung Bean Nuclease
7.5U mung bean nuclease (diluted in mung bean dilution buffer, see end) per 2.5ug time point and incubate at 30° for 30 min. Ethanol ppte, resuspend and load onto a 0.8% TAE gel. Run and excise bands free from undigested DNA. Isolate DNA
Generation of a Nested Set of Deletions Using Exonuclease III
-containing bases incorporated into it. In order to generate a set of insert deletions using Exo III it is necessary to cut the polylinker twice with different restriction enzymes so that the cut end nearest the primer site possesses a 3′ overhang
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