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- 博尔森生物
- BES2084K-1-4PL
- 中国/上海
- 2025年11月26日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
CD40 Knockout U-2OS Cell Ripa Lysate (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
4℃
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
50μL, 2μg/μl
CD40 Knockout U-2OS Cell Ripa Lysate (Pool)
Gene Symbol CD40
Synonyms B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40 antigen(TNF receptor superfamily member 5); CD40 antigen; CD40 molecule; CD40 molecule, TNF receptor
superfamily member 5; CD40 protein.
gRNA Species Human
Parental Cell line U2OS
Knockout Region E3
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 3.
Applications: WB
Packaging 1 vial knockout cell Lysate and 1 vial parental cell Lysate.
Shipping Conditions 4℃
Storage Store at -20°C for 6 months storage or -80°C for long-term storage.
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Predicted to enable antigen binding activity; protein domain specific binding activity; and ubiquitin protein ligase binding activity. Involved in B cell mediated immunity; CD40 signaling pathway; and cellular calcium ion homeostasis. Acts upstream of or
within several processes, including defense response to other organism; positive regulation of B cell activation; and positive regulation of interleukin-12 production. Located in external side of plasma membrane and intracellular membrane-bounded organelle. Part of CD40 receptor complex. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; liver and biliary
system; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); end stage renal disease; hyperimmunoglobulin syndrome (multiple); and non-Hodgkin lymphoma (multiple). Orthologous to human CD40 (CD40 molecule).
Protocol Lysates are made using Ripa buffer with 1mM Phenylmethylsulfonyl fluoride (PMSF).
Proteins in this buffer are denatured.To use as WB control, SDS-PAGE loading buffer should be added to the lysate and boiled for 5 minutes, then loaded with gel.
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文献和实验of chromatin fragmentation, which need to be empirically set up for each cell type employed. Remove medium and add 2ml PBS 1X/1mM PMSF to each plate and scrape cells at room temperature (RT). Pool together the cells from two plates and centrifuge
Studies of the Ubiquitin Proteasome System
) beads (GE Healthcare) 50 mM Tris⋅Cl, pH 7.5 ( appendix 2A ) Putative E3 expressed as a GST fusion in bacteria (GST‐E3; protocol 44 ) Empty bacterial lysate
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for 10 minutes at 4°C to pellet debris. 7. Combine supernatants from the two 1.5 ml centrifuge tubes in a new15 ml conical tube for immunoprecipitation. 8. Save 50 µl of cell lysate from each sample as WCE DNA. Store at -20°C. NOTE: Add protease
