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- 博尔森生物
- BES2070K-1-1P
- 中国/上海
- 2025年11月26日
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- 详细信息
- 文献和实验
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
NOTCH1 Knockout MCF7 Cell Line (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
Dry Ice
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
1-5×10^6/vial
NOTCH1 Knockout MCF7 Cell Line (Pool)
Gene Symbol NOTCH1
Synonyms activated Notch 1; activated Notch-1;Notch homolog 1 translocation associated (Drosophila); NOTCH1; TAN1; Translocation-associated Notch protein TAN-1.
gRNA Species Human
Parental Cell line MCF7
Knockout Region E6
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6.
Number of Cells 1-5×10⁶
Packaging 1 vial knockout cell line and 1 vial parental cell line.
Shipping Conditions Dry Ice
Storage Stored in liquid nitrogen for a long time.
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types.
Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.
Protocol Upon arrival，please resuscitate the cells as soon as possible for culture to confirm cell vitality, status and preservation.
1.Thaw the vial in 37℃ water bath，and gently shake it to accelerate its dissolution.
2.Transfer the cell suspension to a 15mL aseptic centrifuge tube with pre-warmed Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 5mL complete medium，and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3.Discard supernatant after centrifugation.
4.Resuspend the cell pellet with 1mL pre-warmed complete medium, and transferred to a T75 culture flask.
5.Add 15-20mL complete medium.
6.Incubate the culture at 37℃ incubator with 5% CO₂.
7.Cultures should be monitored daily.
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文献和实验Gene Knockout Techniques(1) The Knockout Process
). Spin cell debris to bottom of wells (2000 RPM/2 minutes). Pool by sampling 75 ul from each well of a plate with the Hydra and dispensing 70 ul into a sterile 8-channel pooling block (this looks like a tiny little swimming pool with 8 horizontal lanes
Analysis of B-Cell Signaling Using DT40 B-Cell Line
targeting is conveniently employed to inactivate genes of interest. This chapter describes procedures for (1) generation of knockout DT40 cell line, (2) analysis of surface BCR expression, (3) detection of tyrosine-phosphorylated proteins, (4) detection
Insights into T-Cell Development from Studies Using Transgenic and Knockout Mice
T-cell differentiation is a tightly controlled developmental program observed as the stepwise progression of immature thymocytes through several unique stages characterized by the expression of distinct combinations of cell surface markers
