- ￥7000
- 博尔森生物
- BES2002K-2-2P
- 中国/上海
- 2025年11月26日
5 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
NLRP3 Knockout MCF7 Cell Line (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
Dry Ice
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
1-5×10^6/vial
NLRP3基因敲除MCF7细胞（Pool）
NLRP3 Knockout MCF7 Cell Line (Pool)
Catalog Number BES2002K-2-2PNLRP3 Knockout MCF7 Cell Line (Pool)
Gene Symbol NLRP3
Synonyms LRR and PYD domains-containing protein 3; AGTAVPRL;CIAS1; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLRP3.
Swiss Q96P20
Gene ID 114548
gRNA Species Human
Parental Cell line MCF7
Knockout Region E6
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon 6.
Number of Cells 1-5×10⁶
Packaging 1 vial knockout cell line and 1 vial parental cell line.
Shipping Conditions Dry Ice
Storage Stored in liquid nitrogen for a long time.、
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18.
Protocol Upon arrival，please resuscitate the cells as soon as possible for culture to confirm cell vitality, status and preservation.
1.Thaw the vial in 37℃ water bath，and gently shake it to accelerate its dissolution.
2.Transfer the cell suspension to a 15mL aseptic centrifuge tube with pre-warmed 5mL complete medium，and centrifuge 1000rpm for approximately 5 minutes at room temperature.
3.Discard supernatant after centrifugation.
4.Resuspend the cell pellet with 1mL pre-warmed complete medium, and transferred to a T75 culture flask.
5.Add 15-20mL complete medium.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
6.Incubate the culture at 37℃ incubator with 5% CO₂.
7.Cultures should be monitored daily.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！以实际收货产品说明书为准，网站说明书仅供参考。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
NLRP3 Knockout MCF7 Cell Line (Pool)
￥7000
