2-HYDROXY-5-IMIDAZOL-1-YL-BENZ

单组分溶液配制

, 2.2M dNTP’s 100mM EDTA 0.5M EtBr 10mg/ml Gelatin 2% Glucose 1; 1.5; 2M Guanidine HCl 1-8M HEPES 1M Imidazol 2M Paraformaldehyde 37% PEG 40% PMSF 100mM Retinoic acid 10mM Sucrose 1; 2; 2.5M Tris Cl 1M

Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS

peptides is also described.MATERIALSReagentsAcetic acid (100 mM)Ammonium bicarbonate (50 mM, pH 8.0)Calf intestinal alkaline phosphataseCarboxypeptidase Y (1 µg/µL in H2O)EDTA (100 mM in H2O, pH 8.0)Ferric chloride (FeCl3) (100 mM)Gallium chloride (GaCl

Preparation of Carboxypeptidase Y and Its Properties

】 Carboxypeptidase Y is a kind of proteases in bread yeast. It has the ability to remove most amino acid residue, including that of proline, from the C-termini of proteins and peptides. These unique properties render a useful tool in C-termini