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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
3-[(3,4-DIHYDRO-2H-1,5-BENZODIOXEPIN-7-YLSULFONYL)AMINO]BENZOIC ACID
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
790272-30-9
- 规格:
100mg
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文献和实验(1000X) 0.5 ml + Glutamine (100X) 5 ml + 5% FBS (25 ml) ProcedureDraw venous blood into 60 ml syringe with 10ml 6% dextran + 7 ml citrate/citric acid. Fill to 60 ml total (blood 43 ml).Mix and sediment 30 min at RT (white blood cells now with serum
each with 30 µL of 100 mM acetic acid. To regenerate the columns with either Fe3+ or Ga3+ metal, wash the column three times each with 30 µL of either 60 mM GaCl3 or 100 mM FeCl3. To remove any unbound metal ions, wash the column three times each with 30 µL
Two dimensional peptide mapping
will probably go down. After exposure, cut out the membrane piece of interest and soak it immediately in 0.5% PVP-360 (Polyvinylpyrrolidone, MW360) in 100mM acetic acid for 30 min. at 37°C. If you don't do this, your yield will be poor. This may result
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