alpha-Smooth Muscle Actin Mous

Isolation of Non-muscle Actin

for isolation of Erythrocyte Actin 1. Collect blood from animal using 20 ml/100 ml whole blood acid citrate/dextrose (0.085 M sodium citrate, 0.065 M citric acid 2% dextrose) as anti-coagulate. 2. Centrifuge blood at for 10 min at 4300 x g and remove

Characterization and Validation of Cre‐Driver Mouse Lines

animals Ppm1a‐CreERT2 /RXRαΔAF2(LNL) and Vil1‐CreERT2 /RXRαΔAF2(LNL) mice injected with vehicle (top) or with tamoxifen (bottom). For tamoxifen injections, tamoxifen (Sigma, cat. no. T56648) was prepared at 10 mg/ml in sunflower seed oil (Sigma, cat. no. S

基于PCR技术的染色质沉淀分析

of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx