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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C;避光;避免反复冻融
- 英文名:
Goat Anti-Human IgG Fc (Biotin)
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μg
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
well inserts and cut out mesh insert with a scalpel, forceps and dissecting microscope Place inserts in a 2.0 mL Eppendorf tube with 1 mL of blocking agent (10% goat serum, 1% BSA in PBS). Gently flick tube to mix. Remove solution and wash 3x
antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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