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- 文献和实验
- 技术资料
- 保存条件:
-20°C;避免反复冻融
- 英文名:
PKMYT1 Mouse mAb
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μl
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文献和实验7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
g Stir O/N in cold room. Pour into plastic bottles. Spin at 4 o C, 7,500 rpm for 20 min in JA-10 rotor. Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer . Discard Sup. Resuspend pellets in 10 ml
T-Cell Activation Using mAb to CD3
Add soluble antibody in 100µl to each well. Titrate antibodies for optimal performance in the assay conditions used. If isolated T cells are to be used in proliferation assays, we recommend using these antibodies at 10µg/ml immobilized on plastic (i.e
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