- ￥399
- 源叶
- 中国
- K10413-10μl
- 2025年10月27日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C,避免反复冻融
- 英文名：
Mre11 Mouse mAb
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- 规格：
10μl
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文献和实验) corresponds to about 50µg/ml of chromatin DNA. So the yield of chromatin (in µg) is given by A260 x dilution factor x volume x 50; Centrifuge sample (800 x g for 10 minutes at 4°C) and resuspend pellet to a chromatin DNA concentration of 0.5mg/ml
) MTT (Sigma, cat. no. M5655‐1G) dissolved in PBS at 5 mg/ml. Should be stored in dark and room lights should be off, while working with MTT. DMSO (Fisher, cat. no. D128
Protocol for isolating High Molecular Weight DNA from yolk sacs of 9.5 dpc or later embryos
EDTA, 0.5% SDS to each tube (use 50-100 µl for 10.5 dpc or later embryos). Add 1.6 ml of a fresh 10 mg/ml solution of Proteinase K dissolved in 50 mM Tris (pH 8.0), 1 mM CaCl2 (final conc of 500 µg/ml). 3. Incubate at 50°C several hours to overnight
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