BioLegend 550706 Recombinant M

BioLegend 550706 Recombinant Mouse FLT3L (carrier-free)

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BioLegend 550706 Recombinant Mouse FLT3L (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：550706
产品名称：BioLegend 550706 Recombinant Mouse FLT3L (carrier-free)
产品规格：100 µg

产品说明：
Regulatory Status  RUO
Other Names
FMS-releated tyrosine kinase 3 ligand, FLT3LG, Flt-3 Ligand

描述
小鼠FLT3L最初从小鼠T细胞系P7B-0.3A4克隆；人和小鼠FLT3L蛋白共享72%的氨基酸同一性。FLT3L是作为一种I型膜结合蛋白合成的，它被切割成可溶性生长因子。此外，据报道，FLT3L的可溶性形式是选择性剪接的结果。TACE（ADAM17）在FLT3L的胞外结构域脱落中起关键作用；事实上，TACE缺陷小鼠的血清FLT3L水平降低。FLT3L对树突状细胞（DC）的两个主要亚群的发育至关重要：常规DC（cDC）和浆细胞样DC（pDC）。DC的发育或数量的变化可以改变T细胞的免疫和耐受性。DC和Tregs之间的反馈回路通过FLT3L调节，因为已经表明DC扩增诱导的Tregs增加延迟了小鼠1型自身免疫性糖尿病和IBD的发作。此外，FLT3L促进Treg的形成，从而降低小鼠中抗原诱导的关节炎的严重程度。FLT3L在类风湿性关节炎（RA）患者的滑液中升高，并且FLT3L已被纳入用于预测RA可能发展的临床前标志物组中。疟原虫感染触发的先天感应通路通过FLT3L的释放调节DC稳态和适应性免疫。在疟原虫感染期间，在人类和小鼠中检测到高水平的FLT3L和增加的DC。
Mouse FLT3L was initially cloned from a murine T cell line, P7B-0.3A4; the human and mouse FLT3L proteins share 72% amino acid identity. FLT3L is synthesized as a type I membrane-bound protein, which is cleaved to become a soluble growth factor. Additionally, a soluble form of FLT3L has been reported as a result of alternative splicing. TACE (ADAM17) plays a key role in the ectodomain shedding of FLT3L; in fact, serum FLT3L levels are decreased in TACE deficient mice. FLT3L is crucial for the development of the two main subsets of dendritic cells (DCs): conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Changes in development or the number of DCs can alter T cell immunity and tolerance. A feedback loop between DCs and Tregs is regulated via FLT3L, as it has been shown that the increase in Tregs induced by DC expansion delays the onset of type 1 autoimmune diabetes and IBD in mice. Also, FLT3L facilitates formation of Tregs and thus, reduces severity of antigen-induced arthritis in mice. FLT3L is elevated in the synovial fluid of rheumatoid arthritis (RA) patients and FLT3L has been included in panels of preclinical markers for predicting the possible development of RA. The innate sensing pathway triggered by Plasmodium infection regulates DC homeostasis and adaptive immunity via FLT3L release. High levels of FLT3L and increased DCs have been detected in humans and mice during Plasmodium infection.

Product Details
Source
Mouse FLT3L, amino acids (Gly27-Arg188) (Accession# NM_013520), was expressed in CHO cells.

Molecular Mass
The 162 amino acid recombinant protein has a predicted molecular mass of approximately 18.3 kD. The DTT-reduced and non-reduced protein migrate at approximately 20-26 kD and 24-30 kD by SDS-PAGE. The N-terminal amino acid is Gly.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation 
0.22 µm filtered protein solution is in PBS, pH 6.0.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 5.0 - 25.0 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 105 units/mg, as determined by induction of IL-6 production on M1 cell.
Application   Bioassay

Application Notes
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details
Distribution   Thymic stromal cells, mast cells.
Function
FLT3L stimulates the proliferation of hematopoietic precursors and development of natural killer cells and dendritic cells. FLT3L regulates the homeostatic feedback loop between DCs and Tregs. FcεRI-triggered mast cell degranulation induces an increase of FLT3L in serum.

Interaction
Hematopoietic stem progenitor cells, progenitor cells, NK cells, and DCs.
Ligand/Receptor   RTK Fms-like tyrosine kinase 3 (FLT3).
Bioactivity   Induction of IL-6 on M cells.

Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area    Stem Cells
Molecular Family   Cytokines/Chemokines, Growth Factors

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