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- Sigma
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- SML1311-5MG
- 2025年11月04日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
鹿森生物
- 规格：
5MG
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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 4.3 mM CaCl2,1.2 M KI,50 mM Tris-HCl,pH 8.0,5 ml. 5.0.5 mM ATP,0.2 mM CaCl2,0.5 mM DTT,2 mM Tris-HCl,pH 8.0 at 4℃,1 liter. 6.CFSE (Molecular Probes,C-1311; not the diacetate),prepare a stock solution of 50 mg/ml in DMSO. 7.Small SS34 tubes and adaptors
Labeling Tubulin and Quantifying Labeling Stoichiometry
: For microinjections, we dilute the tubulin in IB to 2-5 mg/ml, clarify by centrifugation and inject ~1/10 of cell volume. For frog extract studies, we add labeled tubulin to 1/40 - 1/200th of the extract tubulin pool (~20 µM). b) Preparation of fluorescent
minutes and the pellets were washed with PBS. After resuspension with 1 ml PBS, the cells were incubated with 10 ml of RNase I (10 mg/ml) and 100 ml of propidium iodide PI (400 mg/ml; Sigma) and shaken for 1 hour at 37ºC in the dark [20]. Samples
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