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- Sigma
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- M6756-100MG
- 2025年10月24日
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- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
鹿森生物
- 规格：
100MG
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文献和实验Sodium Acetate Precipitation of Small Nucleic Acids
sodium acetate. 3. Add 4 volumes of 100% ethanol; i.e. 1 ml 100% ethanol for 230 μl Lower Running Buffer plus 23 μl 3 M sodium acetate. 4. Mix thoroughly and incubate at –20° C overnight (16 hr). 5.
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
serum 1:100 in 3% BSA and 0.02% sodium azide in TBST Transfer diluted serum in a 150 mm Petri dish. Place one membrane in the serum for 1 hour at room temperature, shaking gently. For 30 ml of diluted serum, we usually adsorb 5 membranes
Preparation of High Titer Adenovirus in P11 cells
at �70° in small aliquots. For solutions: (and try to keep everything sterile) Sterile filter 5% Na Deoxycholate and 2 M MgCl2 Autoclave 0.1 M Tris-HCl, pH 8.0 Dnase I solution: Dissolve 100 mg bovine pancreatic
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