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文献和实验The field of mitochondrial dynamics has received a great deal of attention as a result of a number of studies linking mitochondrial fission and fusion machinery to apoptosis. Specifically, elevated levels of mitochondrial fission
Analysis of Fusion Using a Virus-Free Cell Fusion Assay
, we have employed a virus-free cell-cell fusion assay. In the effector cell, the viral glycoproteins and a plasmid containing the T7 promoter, driving the luciferase gene, are expressed. In the target cell type, T7 RNA polymerase is transfected. Fusion
In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase
for initiation at promoter sequences on the mitochondrial DNA template. It has recently become possible to work with highly purified, recombinant forms of the mitochondrial RNA polymerase subunits from yeast. This chapter describes detailed protocols for working
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