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- O2750-10G
- 2025年10月24日
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- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
联硕生物
- 规格：
10G
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文献和实验Sucrose Density Gradient Fractionation
of the 24 hr.culture into 50 ml media).It is helpful to inoculate 2 different flasks--one with 1 ml of the 2 ml culture,the other with 0.5 ml of the 2 ml cultureSpin at 2750 X g.for 10 min.at 30℃Resuspend in 100 ml pre-warmed YPD so that O.D.600 = 0.5 (50 ml
Sucrose Density Gradient Fractionation of Yeast Membranes
of growth) Wait 1 doubling period (about 2 hours) until O.D.600 equals about 1 Add 1 ml of COLD 1M NaN3 to 100 ml culture (we want 10 mM total conc.) Spin down 3 x 109 cells (about 100 O.D. equivalents (100 ml of OD = 1): 2750 X g
--one with 1 ml of the 2 ml culture, the other with 0.5 ml of the 2 ml culture Spin at 2750 X g. for 10 min. at 30oC Resuspend in 100 ml pre-warmed YPD so that O.D.600 = 0.5 (50 ml of O.D.=1 culture into 100 ml total YPD) If appropriate
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