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产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul/200ug
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 规格: | 200ug | 产品价格: | ¥5600.0 |
| 产品编号 | bsm-33006M |
| 英文名称 | VSV-G tag Mouse mAb |
| 中文名称 | VSV-G tag标签单克隆抗体 |
| 英文别名 | Vesicular stomatitis virus glycoprotein tag; VSV epitope tag; VSV tag; VSV-G epitope tag; VSVG; vsvg tag; VSV-G-Tag. |
| 产品应用 | WB=1:1000-5000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated VSV-G tag |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Epitope Tags > Other Tags > VSV-G Tag |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {ELISA} | {1:1000-5000} |

Lane1: VSV-G-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
Lane2: VSV-G-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41230P) at 2ug
Primary: Anti-VSV-G tag (bsm-33006M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD
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文献和实验T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
, and genotype for each mouse that is tagged and tailed. Keep the log sheets with the pedigree. 4. Together, the pedigree, mating cards, and log sheets should provide the following information for each mouse: birth date, sex, ear tag number, coat color, DNA
(MPD) along with genotype information when it is available. References 1. Baron BW, Langan G, Huo D, Baron JM, Montag A. Squamous cell carcinomas of the skin at ear tag sites in aged FVB/N mice. Comp Med. 2005 Jun;55(3):231-5. 2. Cover CE
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