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- bs-3388R
- 2026年10月13日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3388R |
| 英文名称 | phospho-RPS6 (Ser235+Ser236) Rabbit pAb |
| 中文名称 | 磷酸化S6核糖体蛋白抗体 |
| 英文别名 | RPS6(phospho S235+S236); p-RPS6(phospho S235+S236); Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal(1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; RPS 6; RpS6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein. |
| 产品应用 | WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800 Not yet tested in other applications. |
| 交叉反应 | Human, Rat, HepG2 (Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human RPS6 around the phosphorylation site of Ser235/236 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 27 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Ribosome |
| 翻译后修饰 | Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphrylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. |
| 相似性 | Belongs to the ribosomal protein S6e family. |
| 功能 | May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:200-800} |
| {IHC-F} | {1:200-800} |
| {IF} | {1:200-800} |
Hela (H) cells were treated with or without Anisomycin (250ng/ml) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-RPS6 (Ser235+Ser236) polyclonal antibody, unconjugated (bs-3388R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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[IF=9.4] Yingqiang Luo. et al. High-salt microenvironment worsens the progression of atopic dermatitis via activating the SGK–1–mTOR pathway in keratinocytes. GENES DIS. 2026 Mar;:102168 WB Human
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RPS6 (p-S235/S236) Rabbit pAb(bs-3388R)-50ul/100ul/200ul
￥1180 - 2800
