Sample:
Lane 1: Rat Liver tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-Cathepsin D (bs-1615R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11/38/45 kDa
Observed band size: 46 kDa
- ￥1180 - 2800
- Bioss
- bs-1615R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1615R |
| 英文名称 | Cathepsin D Rabbit pAb |
| 中文名称 | 组织蛋白酶D轻链抗体 |
| 英文别名 | Cathepsin D light chain; CatD; CathepsinD; Cathepsin-D; CLN10; CPSD; CTSD; Lysosomal aspartyl peptidase; MGC2311; CATD_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cathepsin D light chain |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 11/38/45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Extracellular matrix Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Cysteine protease > Cathepsins Neuroscience > Cell Adhesion Proteins > Membrane Proteins Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Enzymes > Other Enzymes |
| 亚细胞定位 | Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 组织特异性 | Expressed in the aorta extrcellular space (at protein level). |
| 翻译后修饰 | N- and O-glycosylated. |
| 相似性 | Belongs to the peptidase A1 family. |
| 功能 | Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer and possibly Alzheimer's disease. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 3.51 }}] {Hossain, Shahdat, Hiroyuki Arai, and Osamu Shido. "Neuroprotective Effect of Madecassoside Evaluated Using Amyloid β1-42-Mediated in Vitro and in Vivo Alzheimer’s Disease Models." International Journal of Indigenous Medicinal Plants (2014).} {ELISA} {="Rat"}
[IF={{ 2.74 }}] {Bailey Balouch. et al. Human INCL fibroblasts display abnormal mitochondrial and lysosomal networks and heightened susceptibility to ROS-induced cell death. Plos One. 2021 Feb;16(2):e0239689} {ICC} {Human}
[IF={{ 1.71 }}] {Liao, Peng, et al. "Organellar proteome analyses of ricin toxin-treated HeLa cells." Toxicology and industrial health (2014): 0748233714549066.} {WB} {="Human"}
相关实验
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
,3-glucoronosyltransferase(GlcAT-1)I or S, α-MF,active421Gastric cathepsin ES, 0.6 mg/L, native[231]Gelatinase BS, 50 mg/L, α-MF, active425GlucocerbrosideaseS, α-MF538Glutaminyl cyclaseS, α-MF, active535N-glycosylation enzymesS, I , active549Gp17/GCDFP-15S, 3 mg/L, α-MF
Immunohistochemistry / Immunocytochemistry Procedures
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/96T 2000/3000 进口分装 F0021 骨成型蛋白-9(BMP-9) ELISA 48T/96T 2000/3000 进口分装 F0022 BMP-R1A ELISA 48T/96T 2000/3000 进口分装 F0023 BMP-R2 ELISA 48T/96T 2000/3000 进口分装 F0024 BTC ELISA 48T/96T 2000/3100 进口分装 F0030 CAE-Ab ELISA 96
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Cathepsin D Rabbit pAb(bs-1615R)-50ul/100ul/200ul
￥1180 - 2800
