Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-CD8 antibody (bs-0648R)
Dilution: 2ug/Test;
Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-0648R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0648R |
| 英文名称 | CD8 Rabbit pAb |
| 中文名称 | CD8抗体 |
| 英文别名 | CD8 antigen, alpha polypeptide(p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen. |
| 产品应用 | Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse CD8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 27 kDa |
| 实际分子量 | 32kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > CD markers Immunology > Adaptive Immunity > T Cells > CD Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells |
| 亚基 | In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Most prominently found in the gastrointestinal tract, skin, lung, and brain but not in liver. |
| 相似性 | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| 功能 | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
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文献和实验该产品被引用文献
[IF={{ 9.986 }}] {Christophe Glorieux. et al. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling. Redox Biol. 2021 Jan;38:101780} {IHC} {Mouse}
[IF={{ 9.273 }}] {Qing Yao. et al. Water-responsive gel extends drug retention and facilitates skin penetration for curcumin topical delivery against psoriasis. ASIAN J PHARM SCI. 2023 Feb;:100782} {IF} {Mouse}
[IF={{ 8.9 }}] {Ye Jin. et al. Fluoride-induced osteoporosis via interfering with the RANKL/RANK/OPG pathway in ovariectomized rats: Oophorectomy shifted skeletal fluorosis from osteosclerosis to osteoporosis. ENVIRON POLLUT. 2023 Nov;336:122407} {IHC} {Rat}
[IF={{ 8.806 }}] {Li TF et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.Biomaterials. 2018 Oct;181:35-52.} {IHC} {Human}
[IF={{ 8.39 }}] {Wang, Raymond M., et al. "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." Biomaterials (2017).} {IHC} {="Mouse"}
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CD8 Rabbit pAb(bs-0648R)-50ul/100ul/200ul
￥1180 - 2800
