Sample:
Eye (Mouse) Lysate at 40 ug
Primary: Anti- PPAR gamma (bsm-33436M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
- ￥1180 - 5600
- Bioss
- bsm-33436M
- 2025年10月24日
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- 规格：
50ul/100ul/200ul/200ug
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-33436M |
| 英文名称 | PPAR gamma Mouse mAb |
| 中文名称 | 过氧化酶活化增生受体γ单克隆抗体 |
| 英文别名 | GLM1; CIMT1; NR1C3; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator activated nuclear receptor gamma variant 1; Peroxisome proliferator activated receptor gamma 1; Peroxisome Proliferator Activated Receptor gamma; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARG_HUMAN; PPAR-γ; PPAR γ; PPARγ; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Mouse |
| 免疫原 | KLH conjugated synthetic peptide derived from human PPAR gamma |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 57 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Metabolism Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Fatty acids Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Metabolism > Types of disease > Obesity Neuroscience > Neurology process > Metabolism Stem Cells > Mesenchymal Stem Cells > Adipogenesis |
| 亚基 | Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with AKAP13, LPIN1 and PRDM16. Also interacts with PPARBP coactivator in vitro. Interacts with CITED2; the interaction stimulates its transcriptional activity (By similarity). Interacts with NCOA3 and NCOA6 coactivators. Interacts with ASXL1 AND ASXL2. |
| 亚细胞定位 | Nuclear |
| 组织特异性 | Skeletal muscle, liver, heart and kidney. |
| 相似性 | Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. |
| 功能 | Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Liver (Mouse) Tissue Lysate at 40 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-PPAR gamma (bsm-33436M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 50 kD
Lane 1: Liver (Mouse) Tissue Lysate at 40 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-PPAR gamma (bsm-33436M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPAR gamma) Monoclonal Antibody, Unconjugated (bsm-33436M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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文献和实验该产品被引用文献
[IF={{ 9.685 }}] {Wang, Nan. et al. USP7- and PRMT5-dependent G3BP2 stabilization drives de novo lipogenesis and tumorigenesis of HNSC. CELL DEATH DIS. 2023 Mar;14(3):1-13} {WB} {Human}
[IF={{ 7.5 }}] {Liu Tingjun. et al. Menin orchestrates hepatic glucose and fatty acid uptake via deploying the cellular translocation of SIRT1 and PPARγ. CELL BIOSCI. 2023 Dec;13(1):1-20} {IF} {Mouse}
[IF={{ 6.8 }}] {Han, Ziping. et al. MicroRNA-193a-5p Rescues Ischemic Cerebral Injury by Restoring N2-Like Neutrophil Subsets. TRANSL STROKE RES. 2022 Jul;:1-19} {IF} {Mouse}
[IF={{ 5.6 }}] {Qicai Liu. et al. Adropin deficiency worsens TNBS-induced colitis. INT IMMUNOPHARMACOL. 2023 Nov;124:110891} {IF} {Mouse}
[IF={{ 5 }}] {Jian Lin. et al. IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγ. EUR J PHARMACOL. 2023 Nov;959:176059} {IF,WB} {Mouse}
相关实验
Adipose Tissue-Specific PPAR Gene Targeting
The nuclear receptor peroxisome proliferator activated receptor gamma (PPARγ) is most abundantly expressed in adipose tissue and has been shown to play imperative roles in controlling adipogenesis and lipogenesis in cultured
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PPAR gamma Mouse mAb(bsm-33436M)-50ul/100ul/200ul/200ug
￥1180 - 5600
