Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Rat Kidney tissue lysates
Lane 4: Rat Heart tissue lysates
Lane 5: Pig Liver tissue lysates
Primary: Anti-CAT (bs-2302R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 60 kDa
- ￥1180 - 2800
- Bioss
- bs-2302R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2302R |
| 英文名称 | CAT Rabbit pAb |
| 中文名称 | 过氧化氢酶抗体 |
| 英文别名 | CATA_HUMAN; Catalase; EC:1.11.1.6. |
| 中文别名 | 催化酶; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Pig, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | Catalase natural protein from pig liver |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Cardiovascular > Heart > Cardiac metabolism Metabolism > Pathways and Processes > Redox metabolism > Antioxidants Signal Transduction > Protein Trafficking > Chaperones > Heat Shock Proteins Tags & Cell Markers > Subcellular Markers > Organelles > Peroxisome |
| 亚基 | Homotetramer. |
| 亚细胞定位 | Peroxisome. |
| 翻译后修饰 | The N-terminus is blocked. |
| 相似性 | Belongs to the catalase family. |
| 功能 | Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 5.23 }}] {Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.} {WB} {="Pig"}
[IF={{ 4.2 }}] {Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).} {IHC} {="Mouse"}
[IF={{ 3.85 }}] {Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).} {IHC} {="Sheep"}
[IF={{ 3.69 }}] {Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873} {WB} {Human}
相关实验
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Western blotting using ECL reagent
REAGENTSECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples).Hybond ECL nitrocellulose
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CAT Rabbit pAb(bs-2302R)-50ul/100ul/200ul
￥1180 - 2800
