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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
100ML

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文献和实验Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
. Lysozyme/RNase digestion 1-Add 1 ml per well EC lysis solution without lysozyme and RNase. EC lysis solution: 6 mM tris-HCl (pH 7.6) 1 M NaCl 100 mM EDTA 0.5% Brij-58 0.2% deoxycholate 0.5% Sarkosyl 1 mg/ml lysozyme 20 µg/ml RNase
- 150 micron (medium) and transfer to a sterile bottle. 2. Add 100 ml of sterile water. 3. Let it stand at room temperature for 3 hours or place at 4℃ overnight. Packing and using the columns: 1. Sephadex settles
Bead diameter: 50 - 150 micron (medium) and transfer to a sterile bottle. 2. Add 100 ml of sterile water. 3. Let it stand at room temperature for 3 hours or place at 4℃ overnight. Packing and using the columns: 1. Sephadex settles
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