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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose
attempted affinity methods. Our laboratory has used Reactive Red-agarose in the purification of m-and μ-calpain from bovine heart and other tissues for many years (1 ,2 ). However, this method has not achieved widespread use due to reported variability
TUNEL PROCEDURE IN BOVINE EMBRYOS
Materials 8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C � do not go higher). Add a few drops of 2 N
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