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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
见标签
- 库存:
999
- 供应商:
联硕生物
- 规格:
100MG

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文献和实验Crystallization of Kinesin Family Motor Proteins
Tris pH 7.9, 5 mM MgCl2, 0.5 mM ADPReservoir: 17.5% PEGMME 2000, 100 mM HEPES pH 6.5-7.5, 3% glycerolSong et al., 2001Kar3 +N11 (WT)Hanging Drop 18°C2 microliters of protein (15 mg/ml) + 2 microliters of well solution containing 20-26% PEG2000ME, 0.2 M
- 2.6% - Collagen - - - - 1.6 mg/ml - - 0.1N NaOH - - - - 0.12% - - HEPES - - - - 10% - - 7.5% Sodium bicarbonate - - - - 0.8% - - PureCol - - - - 50%
Fast and reliable mini-prep RNA extraction from Neurospora crassa
, mycelia from plates (4 days old) and single colonies of N. crassa grown on a sorbose plate. With this protocol, from 20 to 300 mg (dry weight) of mycelium, grown in liquid media or on plates, yielded between 200 µg and 3 mg of RNA depending on the growth
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