- ￥99 - 999
- Sigma
- 进口
- 27088-10MG-F
- 2025年10月23日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
见标签
- 库存：
999
- 供应商：
鹿森生物
- 规格：
10MG
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Purification of DNA from lambda phage
tube,extract with phenol again.You can do a third phenol extraction if there is still a lot of white denatured protein at the interface. E.Add 200 III CHCOC3,shake,microcentrifuge briefly.Transfer aqueous layer to new tube,repeat. F.Add 20 jil of 3M
C. add 375 ul CHC13 and shake for 15 min. D. Transfer to round-bottom polypropylene tube, leaving behind the chloroform. E. Centrifuge 10 min @10,000 rpm in 870 rotor on IEC at 4°. F. Decant supernatant to 50ml conical
Construction of BAC Libraries：Construction of a BAC library
without the restriction enzyme on ice for 20 minutes. d. Dilute the Hind III enzyme serially ranging from 0 to 20 units in 1X Hind III reaction buffer. f. Incubate the reactions in a 37°C water bath for 5 minutes and stop the reaction by addition of 1/10
技术资料暂无技术资料 索取技术资料
