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文献和实验Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
Tris-Cl 6g Water to 2 L, pH 7.4-7.5 1xTRIzol: 200ml Final Add Guanidine thiocyanate 0.8 M FW 118.2 18.912 g Ammonium thiocyanate 0.4 M FW 76.12 6.09 g Sodium Acetate 0.1 M, pH 5 Stock 1M 20 ml Glycerol 5% 10ml Add water to 100ml Mix
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RNA
samples in 200 ul 0.5%NP-40/TD by pipetting 10 times again. 18: Follow the steps from 7 to 15 again. Solutions: TD: 2L NaCl 16.0g KCl 0.76g Na2 HPO4 0.20g Tris-Cl 6g Water to 2 L, pH 7.4-7.5 1xTRIzol: 200ml Final Add Guanidine
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RN
off supernatant and resuspend samples in 200 ul 0.5%NP-40/TD by pipetting 10 times again. 18: Follow the steps from 7 to 15 again. Solutions: TD: 2L NaCl 16.0g KCl 0.76g Na2 HPO4 0.20g Tris-Cl 6g Water to 2 L, pH 7.4-7.5 1xTRIzol: 200ml Final
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