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文献和实验Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
Phosphoamino acid analysis:Mark Kamps method
standards (about 0.3 µg each of PSer, PThr, and PTyr) on a cellulose thin layer chromatography plate and then spot your sample. We use 0.1 mm EM cellulose plates You can spot the whole sample if you are skillful and have no choice because you don't
Hints and precautions for the care, feeding and breeding of Neurospora
and supplements. The synthetic crossing medium (SC) of Westergaard and Mitchell is most widely used. The need to adjust pH of SC to 6.5 can be avoided by substituting 0.7 g K2HP04 and 0.5 g KH2HP04 per liter for the monobasic salt in the original formula (1963 N.N
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