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文献和实验until they can be delivered to the reference laboratory. Zeus Medium, a commercial product, is probably similar. 1.0 M potassium citrate buffer pH 7.0: dissolve 21.0 g citric acid monohydrate (or 19.2 g citric acid anhydrous) in 30 mL of hot deionized or distilled
% Bromoacetic Acid Acros Organics 200000-106 99% 4-Methylpiperidine Sigma Aldrich
citrate phosphate dextrose and dilute the solution 1:1 with Hank's buffered salt solution (without CaCl2 , MgCl2 , MgSO4 ) and layer on equal volumes of Histopaque to create well-defined layers. 4) Centrifuge (30 min, 740 g, low break
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