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- 保存条件:
常温
- 保质期:
根据瓶身LOT号查询
- 英文名:
Cesium chloride
- 库存:
有现货
- 供应商:
浙江羽翔生物科技有限公司
- CAS号:
7647-17-8
- 规格:
100G
属性
等级
anhydrous
质量水平
200
产品线
ReagentPlus®
Redi-Dri™
方案
99.9%
表单
powder or crystals
质量
free-flowing
pH值(酸碱度)
6-7.5
mp
645 °C (lit.)
SMILES字符串
[Cl-].[Cs+]
InChI
1S/ClH.Cs/h1H;/q;+1/p-1
InChI key
AIYUHDOJVYHVIT-UHFFFAOYSA-M
一般描述
应用
特点和优势
- 提高效率——免除需要松散硬化粉末的时间和精力
- 更加安全——避免使用工具大力敲碎团块的风险
- 更加经济——制备和溶剂化速度更快,提高效率并节约成本
- 质量保证——经过专业检测的出色品质,无抗结剂添加
- 灵活的用量选择——有从研究到放大级别的用量规格可供选择
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文献和实验DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks.
The present study demonstrates that topoisomerase 3B (TOP3B) forms both RNA and DNA cleavage complexes (TOP3Bccs) in vivo and reveals a pathway for repairing TOP3Bccs. For inducing and detecting cellular TOP3Bccs, we engineer a "self-trapping" mutant of TOP3B (R338W-TOP3B). Transfection with R338W-TOP3B induces R-loops, genomic damage, and growth defect, which highlights the importance of TOP3Bcc repair mechanisms. To determine how cells repair TOP3Bccs, we deplete tyrosyl-DNA phosphodiesterases (TDP1 and TDP2). TDP2-deficient cells show elevated TOP3Bccs both in DNA and RNA. Conversely, overexpression of TDP2 lowers cellular TOP3Bccs. Using recombinant human TDP2, we demonstrate that TDP2 can process both denatured and proteolyzed TOP3Bccs. We also show that cellular TOP3Bccs are ubiquitinated by the E3 ligase TRIM41 before undergoing proteasomal processing and excision by TDP2.
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