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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Porcine Lamina Propria Leucocytes.
- 亚型:
IgM
- 形态:
Tissue Culture Supernatant - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
S/N
- 适应物种:
Pig
- 保质期:
自发货之日起 12 个月
- 目录编号:
MIL3
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 抗体名:
SWC8 antibody | MIL3
- 规格:
2 ml
Mouse anti Pig SWC8, clone MIL3, recognizes the porcine SWC8 cell surface antigen, an antigen that as yet has no identified human homolog. SWC8 is expressed by granulocytes, B cells, a subset of T cells and by some non-haematopoietic cells. Monocytes however do not express SWC8.
Clone MIL3 has been used in two colour flow cytometry with Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218GA) to distinguish between monocytes and granulocytes (Haverson et al. 1994).
Clone MIL3 has been used in two colour flow cytometry with Mouse anti Porcine CD14 antibody, clone MIL2 (MCA1218GA) to distinguish between monocytes and granulocytes (Haverson et al. 1994).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for SWC8 antibody
- Haverson, K. et al. (1994) Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues.
J Immunol Methods. 170 (2): 233-45. - Summerfield, A. et al. (2001) Induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus.
J Gen Virol. 82 (Pt 6): 1309-18. - Chen, L. et al. (2003) Macrophages and MHC class II positive dendritiform cells in the iris and choroid of the pig.
Curr Eye Res. 26: 291-6. - Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
Immunology. 110: 440-9. - Barnard, A.L. et al. (2005) Immune response characteristics following emergency vaccination of pigs against foot-and-mouth disease.
Vaccine. 23: 1037-47. - Zelnickova, P. et al. (2008) Age-dependent changes of proinflammatory cytokine production by porcine peripheral blood phagocytes.
Vet Immunol Immunopathol. 124: 367-78. - Ondrackova, P. et al. (2010) Porcine mononuclear phagocyte subpopulations in the lung, blood and bone marrow: dynamics during inflammation induced by Actinobacillus pleuropneumoniae.
Vet Res. 41: 64. - LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22.
Further Reading
- Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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Mouse anti Pig SWC8,clone MIL3
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