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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Rabbit thymocytes.
- 亚型:
IgG2a
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Rabbit
- 保质期:
自发货之日起 12 个月
- 目录编号:
KEN-4
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, F, IP
- 浓度:
IgG concentration 1.0 mg/ml
- 抗体名:
CD4 antibody | KEN-4
- 规格:
0.1 mg
Mouse anti Rabbit CD4 antibody, clone KEN-4 recognizes the rabbit CD4 cell surface antigen, also known as T-cell surface antigen T4/Leu-3. Rabbit CD4 is a 434 amino acid, with an additional N-terminal signal peptide ~50 kDa cell surface single pass, type I transmembrane glycoprotein expressed by T helper cells.
Mouse anti Rabbit CD4 antibody, clone KEN-4 blocks the allogeneic mixed lymphocyte reaction response.
Mouse anti Rabbit CD4 antibody, clone KEN-4 blocks the allogeneic mixed lymphocyte reaction response.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/25 | 1/200 |
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
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文献和实验该产品被引用文献
Source Reference
- Kotani, M. et al. (1993) Generation and characterization of monoclonal antibodies against rabbit CD4, CD5 and CD11a antigens.
J Immunol Methods. 157 (1-2): 241-52.
References for CD4 antibody
- Perosa, F. and Dammacco, F. (1994) Anti-idiotypic monoclonal antibodies (mAb) to an anti-CD4 mAb induce CD4+ T cell depletion in rabbit.
Int J Clin Lab Res. 24: 208-12. - Renaux, S. et al. (2003) Dynamics and responsiveness of T-lymphocytes in secondary lymphoid organs of rabbits developing immunity to Eimeria intestinalis.
Vet Parasitol. 110 (3-4): 181-95. - Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLos ONE 3: e1627. - Pakandl, M. et al. (2008) Dependence of the immune response to coccidiosis on the age of rabbit suckling.
Parasitol Res. 103 (6): 1265-71. - Yang, J. et al. (2009) Expression and localization of rabbit B-cell activating factor (BAFF) and its specific receptor BR3 in cells and tissues of the rabbit immune system.
Dev Comp Immunol. 33 (5): 697-708. - Chentoufi, A.A. et al. (2010) A novel HLA (HLA-A*0201) transgenic rabbit model for preclinical evaluation of human CD8+ T cell epitope-based vaccines against ocular herpes.
J Immunol. 184: 2561-71. - Rütgen, B.C. et al. (2014) Exploratory assessment of CD4+ T lymphocytes in brown hares (Lepus europeus) using a cross-reactive anti-rabbit CD4 antibody.
Vet Immunol Immunopathol. 161 (1-2): 108-15. - Parameswaran, N. et al. (2014) The A2 gene of alcelaphine herpesvirus-1 is a transcriptional regulator affecting cytotoxicity in virus-infected T cells but is not required for malignant catarrhal fever induction in rabbits.
Virus Res. 188: 68-80.
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Mouse anti Rabbit CD4,clone KEN-4
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