- 询价
- BIO-RAD
- MCA2120
- 2025年10月17日
- C, E, F *, IF, IP
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Purified human Granzyme B.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
GB11
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, E, F *, IF, IP
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
Granzyme B antibody | GB11
- 规格：
0.1 mg
Mouse anti Granzyme B antibody, clone GB11 recognizes the serine protease Granzyme B, important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).
Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death.
Mouse anti Granzyme B antibody, clone GB11 is suitable for the detection of Granzyme B expressing cells by flow cytometry. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B.
Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death.
Mouse anti Granzyme B antibody, clone GB11 is suitable for the detection of Granzyme B expressing cells by flow cytometry. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 2ug/ml | 5ug/ml |
| Flow Cytometry 1 | ✓ | 1/10 | 1/100 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
- ELISA
- Mouse anti Human granzyme B antibody, clone GB11 may be used as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with biotin conjugated clone GB10 (MCA2119B) as a detection reagent.
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文献和实验该产品被引用文献
Source Reference
- Hsi, B.L. & Yeh, C.J. (1986) Monoclonal antibodies to human amnion.
J Reprod Immunol. 9 (1): 11-21.
References for Granzyme B antibody
- Spaeny-Dekking, E.H. et al. (1998) Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo.
J Immunol. 160 (7): 3610-6. - Wever, D. C. et al. (1998) The CD8+ Granzyme B+ T cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells.
Immunol. 93: 383 - Mahrus, S. & Craik, C.S. (2005) Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells.
Chem Biol. 12: 567-77. - Tschopp, C.M. (2006) Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma.
Blood.108:2290-9. - Schleypen, J.S. et al. (2006) Cytotoxic markers and frequency predict functional capacity of natural killer cells infiltrating renal cell carcinoma.
Clin Cancer Res. 12: 718-25. - Hallermalm, K. et al. (2008) Modulation of the tumor cell phenotype by IFN-gamma results in resistance of uveal melanoma cells to granule-mediated lysis by cytotoxic lymphocytes.
J Immunol. 180: 3766-74. - Clayton, A. et al. (2008) Human tumor-derived exosomes down-modulate NKG2D expression.
J Immunol. 180: 7249-58. - Hufner, K. et al. (2009) Fewer latent herpes simplex virus type 1 and cytotoxic T cells occur in the ophthalmic division than in the maxillary and mandibular divisions of the human trigeminal ganglion and nerve.
J Virol. 83: 3696-703.
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Mouse anti Granzyme B，clone GB11
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