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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Equine thymocytes.
- 亚型:
IgG1
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Horse
- 保质期:
自发货之日起 12 个月
- 目录编号:
CVS4
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, F, IP
- 浓度:
IgG concentration 1.0mg/ml
- 抗体名:
CD4 antibody | CVS4
- 规格:
0.1 mg
Mouse anti Horse CD4 antibody, clone CVS4 recognizes equine CD4, a ~58 kDa cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells
A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).
In addition to the CVS4 clone, other CVS clones recognizing equine cell surface and MHC antigen are available from Bio-Rad.
A study undertaken using Mouse anti Horse CD4, clone CVS4 to identify CD4 on several wild african equid species indicates that the CVS4 clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).
In addition to the CVS4 clone, other CVS clones recognizing equine cell surface and MHC antigen are available from Bio-Rad.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/25 | 1/200 |
| Immunohistology - Frozen | ✓ | ||
| Immunoprecipitation | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD4 antibody
- Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
Immunology. 74 (2): 251-7. - Kydd, J. et al. (1994) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991.
Vet Immunol Immunopathol. 42 (1): 3-60. - Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62: 101-143 - Roberto Da Costa, R.P. et al. (2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.
Reprod Domest Anim. 38 (6): 464-9. - Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses.
Invest Ophthalmol Vis Sci. 45: 2286-92 - Ferreira-Dias, G. et al. (2005) Seasonal reproduction in the mare: possible role of plasma leptin, body weight and immune status.
Domest Anim Endocrinol. 29 (1): 203-13. - Garcia-Tapia, D. et al. (2006) Replication of West Nile virus in equine peripheral blood mononuclear cells.
Vet Immunol Immunopathol. 110 (3-4): 229-44. - Goodman, L.B. et al. (2007) A point mutation in a herpesvirus polymerase determines neuropathogenicity.
PLoS Pathog. 3(11):e160.
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Mouse anti Horse CD4,clone CVS4
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