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- 详细信息
- 技术资料
- 免疫原:
A peptide comprising amino acids 1-39 of mouse AATF (apoptosis-antagonizing transcription factor)
- 亚型:
IgG2a
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
1B2D8
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
IF, WB
- 浓度:
IgG concentration 1.0 mg/ml
- 抗体名:
AATF antibody | 1B2D8
- 规格:
0.1 mg
Rat anti Mouse AATF antibody, clone 1B2D8 recognizes apoptosis-antagonizing transcription factor, also known as Rb-binding protein che-1.
AATF was first identified as an RNA pol II – interacting factor (Fanciulli et al. 2000). AATF is a protein encoded by the AATF gene. AATF mediates cellular responses and promotes cell proliferation and survival by inducing autophagy, DNA repair, and cell cycle arrest and inhibiting apoptosis (Kumar et al. 2019). DNA damage leads to phosphorylation of AATF at tyrosine 366 by the MAP kinase-activated protein kinase 2 (MK2) (Höpker et al. 2012).
Rat anti Mouse AATF antibody detects a band of 80 kDa.
AATF was first identified as an RNA pol II – interacting factor (Fanciulli et al. 2000). AATF is a protein encoded by the AATF gene. AATF mediates cellular responses and promotes cell proliferation and survival by inducing autophagy, DNA repair, and cell cycle arrest and inhibiting apoptosis (Kumar et al. 2019). DNA damage leads to phosphorylation of AATF at tyrosine 366 by the MAP kinase-activated protein kinase 2 (MK2) (Höpker et al. 2012).
Rat anti Mouse AATF antibody detects a band of 80 kDa.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | ✓ | 1/100 | 1/500 |
| Western Blotting | ✓ | 2 ug/ml | 4 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Rat anti Mouse AATF,clone 1B2D8
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