- 询价
- BIO-RAD
- OBT1610
- 2025年10月17日
- C, P, WB
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
10C2
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, P, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
Nestin antibody | 10C2
- 规格：
0.1 mg
Mouse anti Human Nestin antibody, clone 10C2 recognizes nestin, a large intermediate filament protein originally identified by (Hockfield and McKay 1985). Nestin is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | ✓ | ||
| Immunohistology - Paraffin | ✓ | 1/50 | 1/200 |
| Western Blotting | ✓ | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Suggested positive control: WI-38 or U251 cell lysate.
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文献和实验该产品被引用文献
References for Nestin antibody
- Martínez-Navarrete, G.C. et al. (2008) Gradual morphogenesis of retinal neurons in the peripheral retinal margin of adult monkeys and humans
J Comp Neurol. 511: 557-80. - González-Garza, M.T. et al. (2013) Differentiation of CD133+ stem cells from amyotrophic lateral sclerosis patients into preneuron cells.
Stem Cells Transl Med. 2 (2): 129-35. - Aljammal, K. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades
J Cytol Histol. 6:313. - Sterlacci, W. et al. (2014) Putative stem cell markers in non-small-cell lung cancer: a clinicopathologic characterization.
J Thorac Oncol. 9 (1): 41-9. - Nowak, A. et al. (2017) Nestin-positive microvessel density is an independent prognostic factor in breast cancer.
Int J Oncol. 51 (2): 668-76. - Arranz, L. et al. (2014) Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms.
Nature. 512 (7512): 78-81. - Del Toro, R. et al. (2016) Nestin(+) cells direct inflammatory cell migration in atherosclerosis.
Nat Commun. 7: 12706. - Forte, D. et al. (2020) Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy.
Cell Metab. 32 (5): 829-843.e9.
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文献支持
Mouse anti Human Nestin，clone 10C2
询价
