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- 技术资料
- 免疫原:
Purified Pekin duck yolk IgY.
- 亚型:
IgG1
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Duck
- 保质期:
自发货之日起 12 个月
- 目录编号:
16C7
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
E, F, WB
- 浓度:
IgG concentration 0.5mg/ml
- 抗体名:
IgY antibody | 16C7
- 规格:
0.25 mg
Mouse anti Duck IgY antibody, clone 16C7 recognises both the IgY and IgY(-Fc) Pekin duck heavy chain molecules.
The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. The IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab′)2 fragment of IgY.
Mouse anti Duck IgY antibody, clone 16C7 also detects IgY heavy chain in Mallard ducks, goose and swan.
Mouse anti Duck IgY antibody, clone 16C7 detects bands of approximately 118 kDa and 178-200 kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.
The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. The IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab′)2 fragment of IgY.
Mouse anti Duck IgY antibody, clone 16C7 also detects IgY heavy chain in Mallard ducks, goose and swan.
Mouse anti Duck IgY antibody, clone 16C7 detects bands of approximately 118 kDa and 178-200 kDa in Pekin duck cell lysates under non-reducing conditions in Western blotting.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | ||
| Flow Cytometry | ✓ | ||
| Western Blotting | ✓ | non-reducing |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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文献和实验该产品被引用文献
Further Reading
- Magor, K.E. et al. (1994) One gene encodes the heavy chains for three different forms of IgY in the duck.
J Immunol. 153 (12): 5549-55. - Higgins, D.A. & Warr, G.W. (1993) Duck immunoglobulins: structure, functions and molecular genetics.
Avian Pathol. 22 (2): 211-36.
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Mouse anti Duck IgY Heavy Chain,clone 16C7
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