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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mouse spleen cells.
- 亚型:
IgG2a
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
YTS105.18
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, F, IF
- 浓度:
IgG concentration 1 mg/ml
- 抗体名:
CD8 Alpha antibody | YTS105.18
- 规格:
0.25 mg/25 µg
| 规格: | 0.25 mg | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 25 µg | 产品价格: | 询价 |
Rat anti Mouse CD8, clone YTS105.18 recognizes a non polymorphic epitope on the mouse CD8 alpha chain. This antibody has been reported to block MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies (Cobbold et al. 1990 & Wise et al. 1998).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | 1/50 | 1/100 |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD8 Alpha antibody
- Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
Eur J Immunol. 20 (12): 2747-55. - Qin, S.X. et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies.
Eur J Immunol. 20 (12): 2737-45. - Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
J Immunol. 161 (11): 5813-6. - Sroga, J.M. et al. (2003) Rats and mice exhibit distinct inflammatory reactions after spinal cord injury.
J Comp Neurol. 462: 223-40. - Himoudi, N. et al. (2007) Development of anti-PAX3 immune responses; a target for cancer immunotherapy
Cancer Immunol Immunother. 56: 1381-95. - Lacroix-Lamande, S. et al. (2009) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
PLoS One. 4: 1-8. - Karlsson, M.R. et al. (2010) Hypersensitivity and oral tolerance in the absence of a secretory immune system.
Allergy. 65: 561-70. - Nakashima, H. et al. (2011) A Novel Combination Immunotherapy for Cancer by IL-13Rα2-Targeted DNA Vaccine and Immunotoxin in Murine Tumor Models.
J Immunol. 187: 4935-46.
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Rat anti Mouse CD8,clone YTS105.18
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