- 询价
- BIO-RAD
- MCA1782EL
- 2025年10月17日
- E, ES, F *, FN, WB
- Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant bovine IL-12.
- 亚型：
IgG2a
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Low Endotoxin
- 适应物种：
Bovine
- 保质期：
自发货之日起 12 个月
- 目录编号：
CC301
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
E, ES, F *, FN, WB
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
IL-12 / IL-23 antibody | CC301
- 规格：
0.5 mg
Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 recognizes the p40 subunit of bovine interleukin-12, and binds to both the free subunit and the intact heterodimer. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A.
Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 may be used as a capture antibody in an ELISA in combination with biotinylated Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 (MCA2173B) as a detection reagent.
Mouse anti Bovine Interleukin-12/23 antibody, clone CC301 may be used as a capture antibody in an ELISA in combination with biotinylated Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 (MCA2173B) as a detection reagent.
储存信息
Store at -20°C only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 8ug/ml | |
| ELISpot | ✓ | ||
| Flow Cytometry 1 | ✓ | 1/10 | |
| Functional Assays | ✓ | 1/100 | |
| Western Blotting | ✓ | 1/50 | 1/100 |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- ELISA
- This antibody may be used as a capture antibody in a sandwich ELISA in combination with biotinylated clone CC326 (MCA2173B) as detection reagent (Bannerman et al. 2004).
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文献和实验该产品被引用文献
References for IL-12 / IL-23 antibody
- Hope, J.C. et al. (2002) Development of detection methods for ruminant interleukin (IL)-12.
J Immunol Methods. 266 (1-2): 117-26. - Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. - Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38. - Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. - Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. - Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72. - Davis, T.L. and Pate, J.L. (2007) Bovine luteal cells stimulate proliferation of major histocompatibility nonrestricted gamma delta T cells.
Biol Reprod. 77: 914-22. - Ferret-Bernard, S. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705.
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Mouse anti Bovine Interleukin-12/23:Low Endotoxin，clone CC301
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