- 询价
- BIO-RAD
- MCA2173B
- 2025年10月17日
- E
- Bovine
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant bovine IL-12.
- 亚型：
IgG2b
- 形态：
Purified IgG conjugated to biotin - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Biotin
- 适应物种：
Bovine
- 保质期：
自发货之日起 12 个月
- 目录编号：
CC326
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
E
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
IL-12 / IL-23 antibody | CC326
- 规格：
0.25 mg
Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 recognizes the p40 subunit of bovine interleukin-12. The p40 subunit is also known as IL-12B and can form a heterodimer with either IL-12A or IL-23A. Mouse anti Bovine Interleukin-12/23 antibody, clone CC326 has been shown to block the biological activity of bovine IL-12.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | 5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells of 100μl
- ELISA
- Biotin conjugated Mouse anti Bovine interleukin-12/23 antibody, clone CC326 may be used as a detection reagent in a sandwich ELISA assay using MCA1782EL as capture reagent (Bannerman et al. 2004).
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文献和实验该产品被引用文献
Source Reference
- Hope, J.C. et al. (2002) Development of detection methods for ruminant interleukin (IL)-12.
J Immunol Methods. 266 (1-2): 117-26.
References for IL-12 / IL-23 antibody
- Stephens, S.A. et al. (2003) Differences in cytokine synthesis by the sub-populations of dendritic cells from afferent lymph.
Immunology. 110: 48-57. - Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. - Bannerman, D.D. et al. (2004) Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection.
Clin Diagn Lab Immunol. 11: 463-72. - Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38. - Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
Vet Res. 42:19. - Contreras, V. et al. (2010) Existence of CD8α-like dendritic cells with a conserved functional specialization and a common molecular signature in distant mammalian species.
J Immunol. 185: 3313-25. - Ferret-Bernard, S. et al. (2010) Cellular and molecular mechanisms underlying the strong neonatal IL-12 response of lamb mesenteric lymph node cells to R-848.
PLoS One. 5: e13705. - Souza, M. et al. (2008) Pathogenesis and immune responses in gnotobiotic calves after infection with the genogroup II.4-HS66 strain of human *.
J Virol. 82: 1777-86.
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文献支持
Mouse anti Bovine Interleukin-12/23:Biotin，clone CC326
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