- 询价
- BIO-RAD
- MCA2320T
- 2025年10月17日
- C, E, F
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant soluble MAdCAM-1-fc fusion protein.
- 亚型：
IgG1
- 形态：
Purified IgG - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
314G8
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
C, E, F
- 浓度：
IgG concentration 1.0 mg/ml
- 抗体名：
MAdCAM-1 antibody | 314G8
- 规格：
25 µg/0.2 mg
| 规格： | 25 µg | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 0.2 mg | 产品价格： | 询价 |
Mouse anti Human MAdCAM-1 antibody, clone 314G8 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyer's patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7 (Leung et al. 2004).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | ||
| Flow Cytometry | ✓ | 1/10 | 1/100 |
| Immunohistology - Frozen | ✓ | 1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for MAdCAM-1 antibody
- Leung, E. et al. (1996) Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts.
Immunol Cell Biol. 74 (6): 490-6. - Leung, E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.
Immunol Cell Biol. 82 (4): 400-9. - Olloquequi, J. et al. (2011) Significant increase of CD57+ cells in pulmonary lymphoid follicles of COPD patients.
Eur Respir J. 37 (2): 289-98. - Guerra-Pérez, N. et al. (2015) Retinoic Acid Imprints a Mucosal-like Phenotype on Dendritic Cells with an Increased Ability To Fuel HIV-1 Infection.
J Immunol. pii: 1402623. - Goode, D. et al. (2014) Sex hormones selectively impact the endocervical mucosal microenvironment: implications for HIV transmission.
PLoS One. 9 (5): e97767. - Guzzo, C. et al. (2017) Virion incorporation of integrin αα4β7 facilitates HIV-1 infection and intestinal homing.
Sci Immunol. 2 (11) [Epub ahead of print].
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文献支持
Mouse anti Human MAdCAM-1，clone 314G8
询价
