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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1.
- 亚型:
IgG2a
- 形态:
Purified IgG - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
Purified
- 适应物种:
Mouse
- 保质期:
自发货之日起 12 个月
- 目录编号:
3/23
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
C, E, F, IF
- 浓度:
IgG concentration 1 mg/ml
- 抗体名:
CD40 antibody | 3/23
- 规格:
0.1 mg/0.25 mg
| 规格: | 0.1 mg | 产品价格: | 询价 |
|---|---|---|---|
| 规格: | 0.25 mg | 产品价格: | 询价 |
Rat anti Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40.
Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | ✓ | ||
| Flow Cytometry | ✓ | 1/10 Pack Size: 0.1 mg 1/50 Pack Size: 0.25 mg |
1/50 Pack Size: 0.1 mg 1/100 Pack Size: 0.25 mg |
| Immunofluorescence | ✓ | ||
| Immunohistology - Frozen | ✓ |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
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文献和实验该产品被引用文献
Source Reference
- Hasbold, J. et al. (1994) Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies.
Eur J Immunol. 24 (8): 1835-42.
References for CD40 antibody
- Parry, S.L. et al. (1994) Plastic-immobilized anti-mu or anti-delta antibodies induce apoptosis in mature murine B lymphocytes.
Eur J Immunol. 24 (4): 974-9. - Hasbold, J. & Klaus, G.G. (1994) B cells from CBA/N mice do not proliferate following ligation of CD40.
Eur J Immunol. 24 (1): 152-7. - Salomon, B. et al. (1998) Three populations of mouse lymph node dendritic cells with different origins and dynamics.
J Immunol. 160: 708-17. - Yang, Y.F. et al. (2000) Requirement for IFN-gamma in IL-12 production induced by collaboration between v(alpha)14(+) NKT cells and antigen-presenting cells.
Int Immunol. 12: 1669-75. - Ogasawara, K. et al. (2002) Profiles of cell-to-cell interaction of Mycobacterium intracellulare-induced immunosuppressive macrophages with target T cells in terms of suppressor signal transmission.
Clin Exp Immunol. 129: 272-80. - Russo, S. et al. (2003) Platelet-activating factor mediates CD40-dependent angiogenesis and endothelial-smooth muscle cell interaction.
J Immunol. 171: 5489-97. - Mukhopadhyay, S. et al. (2004) Activation of murine macrophages by Neisseria meningitidis and IFN-gamma in vitro: distinct roles of class A scavenger and Toll-like pattern recognition receptors in selective modulation of surface phenotype.
J Leukoc Biol. 76: 577-84. - Mohan, J. et al. (2005) Skin-derived dendritic cells acquire and degrade the scrapie agent following in vitro exposure.
Immunology. 116: 122-33.
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Rat anti Mouse CD40,clone 3/23
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