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- BIO-RAD
- MCA594F
- 2025年10月17日
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- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peripheral blood mononuclear cells.
- 亚型：
IgG1
- 形态：
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性：
Monoclonal Antibody
- 标记物：
FITC
- 适应物种：
Human
- 保质期：
自发货之日起 12 个月
- 目录编号：
FMC-25
- 供应商：
伯乐生命医学产品（上海）有限公司
- 应用范围：
F
- 浓度：
IgG concentration 0.1mg/ml
- 抗体名：
CD42a antibody | FMC-25
- 规格：
0.1 mg
Mouse anti Human CD42a antibody, clone FMC-25 recognizes human CD42a, also known as Platelet glycoprotein IX, Glycoprotein 9 or GP-IX. CD42a is a 177 amino acid, ~20kDa type I single pass transmembrane glycoprotein containing a single leucine-rich repeat containing N-terminal domain and a single leucine-rich repeat containing C-terminal domain.
CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yan et al. 2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearance of giant platelets in the circulation (Johns et al. 2014).
Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with antiphospholipid syndrome (Godeau et al. 1997).
CD42a is expressed by platelets and megakaryocytes and forms a covalent complex with CD42c (GP-1b-beta), CD42b (GP-1b-alpha) and CD42d (platelet glycoprotein V) to create the platelet surface receptor for von Willebrand factor. Incubation of the intact von Willebrand receptor complex with clone FMC-25 does not appear to inhibit binding of von Willebrand factor to the receptor (Yan et al. 2011). Defects in the GP1BB gene encoding human CD42a can lead to the inherited bleeding disorder Bernard-Soulier syndrome (Diz-Küçükkaya 2013), characterized by prolonged bleeding times, thrombocytopenia and the appearance of giant platelets in the circulation (Johns et al. 2014).
Mouse anti human CD42a antibody, clone FMC-25 has been successfully used as a capture reagent for platelet-autoantibody complexes in the sera of patients presenting thrombocytopenia associated with antiphospholipid syndrome (Godeau et al. 1997).
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Histology Positive Control Tissue
- Bone marrow
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文献和实验该产品被引用文献
Source Reference
- Zola, H. et al. (1984) Monoclonal antibodies against antigens of the human platelet surface: preparation and properties.
Pathology. 16 (1): 73-8.
References for CD42a antibody
- Berndt, M.C. et al. (1985) Molecular characterization of quinine/quinidine drug-dependent antibody platelet interaction using monoclonal antibodies.
Blood. 66 (6): 1292-301. - Berndt, M.C. et al. (1985) Purification and preliminary characterization of the glycoprotein Ib complex in the human platelet membrane.
Eur J Biochem. 151 (3): 637-49. - Berndt, M.C. et al. (1983) Additional glycoprotein defects in Bernard-Soulier's syndrome: confirmation of genetic basis by parental analysis.
Blood. 62 (4): 800-7. - San Miguel, J.F. et al. (1985) Characterization of blast cells in chronic granulocytic leukaemia in transformation, acute myelofibrosis and undifferentiated leukaemia. II. Studies with monoclonal antibodies and terminal transferase.
Br J Haematol. 59 (2): 297-309. - San Miguel, J.F. et al. (1986) Surface marker analysis in acute myeloid leukaemia and correlation with FAB classification.
Br J Haematol. 64 (3): 547-60. - Smith GA et al. (2007) Severe fetomaternal alloimmune thrombocytopenia due to anti-human platelet antigen (HPA)-1a in a mother with a rare and silenced ITGB3*0101 (GPIIIa) allele.
Vox Sang. 93 (4): 325-30. - Berndt, M.C. et al. (1988) Ristocetin-dependent reconstitution of binding of von Willebrand factor to purified human platelet membrane glycoprotein Ib-IX complex.
Biochemistry. 27 (2): 633-40. - Yan, R. et al. (2011) Reconstitution of the platelet glycoprotein Ib-IX complex in phospholipid bilayer Nanodiscs.
Biochemistry. 50: 10598-606.
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Mouse anti Human CD42a:FITC，clone FMC-25
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