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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Equine leucocytes.
- 亚型:
IgG1
- 形态:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Horse
- 保质期:
自发货之日起 12 个月
- 目录编号:
CVS5
- 供应商:
伯乐生命医学产品(上海)有限公司
- 应用范围:
F
- 浓度:
IgG concentration 0.1 mg/ml.
- 抗体名:
CD5 antibody | CVS5
- 规格:
0.1 mg
Mouse anti Horse CD5 antibody, clone CVS5 recognizes the equine CD5 antigen, a single-pass type I membrane protein with an approximate molecular weight of 69 kDa. Equine CD5 is expressed on the majority of T-lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B-cells and granulocyctes.
In addition to the CVS5 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
In addition to the CVS5 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
储存信息
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | ✓ | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
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文献和实验该产品被引用文献
References for CD5 antibody
- Lunn, D.P. et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets.
Immunology. 74 (2): 251-7. - Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995.
Vet Immunol Immunopathol. 62 (2): 101-43. - Moyo, N.A. et al. (2013) Differentiation and activation of equine monocyte-derived dendritic cells are not correlated with CD206 or CD83 expression.
Immunology. 139 (4): 472-83. - Mayall, S. et al. (2001) The anti-human CD21 antibody, BU33, identifies equine B cells.
J Comp Pathol. 124 :83-7. - Siedek, E. et al. (1997) Isolation and characterisation of equine dendritic cells.
Vet Immunol Immunopathol. 60: 15-31. - Colbath, A.C. et al. (2017) Autologous and Allogeneic Equine Mesenchymal Stem Cells Exhibit Equivalent Immunomodulatory Properties In Vitro.
Stem Cells Dev. 26 (7): 503-11. - Ziegler, A. et al. (2016) Equine dendritic cells generated with horse serum have enhanced functionality in comparison to dendritic cells generated with fetal bovine serum.
BMC Vet Res. 12 (1): 254. - Ziegler, A. et al. (2016) Identification and characterization of equine blood plasmacytoid dendritic cells.
Dev Comp Immunol. 65: 352-7.
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文献支持
Mouse anti Horse CD5:FITC,clone CVS5
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